Effect of alpha-lipoic acid on rat kidney and liver microsomes subjected to oxidative stress induced by ascorbate-Fe++

Metabolic changes during kidney and liver disease can induce increased production of oxygen radicals that play an important role in the progression damage appearance comorbidities. In study reported here, effect alpha-lipoic acid (ALA) on chemiluminescence (CL) TBARS determination microsomes isolated from rat was analyzed. Oxidative stress induced by subjecting samples (1 mg protein) to ascorbate-Fe++-dependent (0.4 mM)-Fe++ (2.15 μM) prooxidant system at 37 °C for 120 min. quantified two methods, CL: CL determined using a Packard 1900 TR liquid scintillation counter (Meriden CT, USA). expressed as cpm (counts per minute) read every 10 minutes establish course peroxidation function time. Likewise, total value (sum readings) used compare inhibitory ALA different concentrations corresponding 0.05; 0.15 0.25 μg microsomal protein. Controls were run simultaneously without addition ascorbate-Fe++. It observed lower obtained group than control (without ALA). addition, -ALA found be concentration-dependent (0.05, solution) reduced CL, measured cpm. After it done lipid products (Thiobarbituric Acid Reactive Substances—TBARS). samples. Lipoperoxidation inhibition depended concentration incubation mixture. The results show all tested, protective oxidative found. level analyzes indicate may act antioxidant protecting lipoperoxidative damage.